Fig. 2

Color transition of ovarian tissues after decellularization by optimal protocol (1% SDS pretreatment + scCO2 at 200 bar and 40 °C for 1.5 h); A Native ovarian tissue cortex, B Decellularized scaffold, C DNA quantification assay demonstrated a significant decrease in the DNA content after using 1% SDS for 4 h as a pretreatment with scCO2 system at 200 bar and 40 °C for 1.5 h. (Data are expressed as the mean ± standard error of the mean (SEM), n = 3 per group)