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Table 1 Experimental protocols

From: Preparation and characterization of human decellularized ovarian scaffold based on supercritical carbon dioxide protocol

 

Protocols

Extraction parameters

Co-solvent

Pretreatment

Phase 1

scCO2

T = 40 °C, P = 200 Bar, t = 1.5 h

T = 40 °C, P = , t = 1.5 h

–

–

Phase 2

scCO2 + Co-solvent

T = 40 °C, P = 200 Bar, t = 1.5 h

Ethanol 70%

–

Pretreatment + scCO2

T = 40 °C, P = 200 Bar, t = 1.5 h

–

t = 4 h

  1. scCO2 supercritical carbon dioxide, SDS sodium dodecyl sulfate, T temperature, P pressure, t time